Publikacje 2018-2025

  • Baran K, Skrzynska K, Czyrek AA, Wittek A, Krowarsch D, Szlachcic A, Zakrzewska M, Chudzian J. Fibroblast Growth Factors in Lung Development and Regeneration: Mechanisms and Therapeutic Potential. Cells. 2025 Aug 14;14(16):1256. doi: 10.3390/cells14161256. PMID: 40862735; PMCID: PMC12384526.
  • Ciura K, Sobota O, Chorążewska A, Krowarsch D, Porębska N, Opaliński Ł. Reprogramming FGF1 from the natural growth factor to the engineered heparan sulphate biosensor. Cell Commun Signal. 2025 May 28;23(1):248. doi: 10.1186/s12964-025-02269-x. PMID: 40437501; PMCID: PMC12121223.
  • Czyrek AA, Baran K, Hruba E, Horackova A, Bosakova V, Chudzian J, Fafilek B, Laskova V, Stepankova V, Bednar D, Karl K, Kasparek P, Bosakova M, Killinger M, Szotkowska T, Prochazka J, Zieba JT, Rico-Llanos G, Fric J, Hadzic S, Loku E, Wujak M, Svozilova K, Stroblova M, Sedlacek R, Hristova K, Krakow D, Kubovciak J, Delattre M, Bartoszewski R, Buchtova M, Krowarsch D, Chaloupkova R, Zakrzewska M, Krejci P. Increased thermal stability of FGF10 leads to ectopic signaling during development. Cell Mol Life Sci. 2025 Apr 21;82(1):167. doi: 10.1007/s00018-025-05681-1. PMID: 40257501; PMCID: PMC12011707.
  • Biadun M, Sidor S, Kalka M, Karelus R, Sochacka M, Krowarsch D, Opalinski L, Zakrzewska M. Production and purification of recombinant long protein isoforms of FGF11 subfamily. J Biotechnol. 2025 Jul;403:9-16. doi: 10.1016/j.jbiotec.2025.03.016. Epub 2025 Mar 26. PMID: 40154621.
  • Zlinska V, Feketova Z, Czyrek A, Chudzian J, Zivkovic ML, Ursachi VC, Dudeja P, Fafilek B, Rynes J, Rico-Llanos G, Koudelka A, Roy T, Biadun M, Raskova V, Svozilova K, Stroblova M, Krzyscik M, Hristova K, Krowarsch D, Foldynova-Trantirkova S, Zakrzewska M, Trantirek L, Krejci P. Specific inhibition of fibroblast growth factor receptor 1 signaling by a DNA aptamer. Mol Ther NucleicAcids. 2024 Nov 28;36(1):102405. doi: 10.1016/j.omtn.2024.102405. PMID:39759879; PMCID: PMC11700292.
  • Szymczyk J, Sochacka M, Biadun M, Sluzalska KD, Witkowska D, Zakrzewska M. Overcoming drug resistance of cancer cells by targeting the FGF1/FGFR1 axis with honokiol or FGF ligand trap. Front Pharmacol. 2024 Sep 12;15:1459820. doi: 10.3389/fphar.2024.1459820. PMID: 39329123; PMCID: PMC11424896.
  • Biadun M, Sochacka M, Kalka M, Chorazewska A, Karelus R, Krowarsch D, Opalinski L, Zakrzewska M. Uncovering key steps in FGF12 cellular release reveals a common mechanism for unconventional FGF protein secretion. Cell Mol Life Sci. 2024 Aug 19;81(1):356. doi: 10.1007/s00018-024-05396-9. PMID:39158730; PMCID: PMC11335280.
  • Gędaj A, Chorążewska A, Ciura K, Karelus R, Żukowska D, Biaduń M, Kalka M, Zakrzewska M, Porębska N, Opaliński Ł. The intracellular interplay between galectin-1 and FGF12 in the assembly of ribosome biogenesis complex. Cell Commun Signal. 2024 Mar 11;22(1):175. doi: 10.1186/s12964-024-01558-1. PMID: 38468333; PMCID: PMC10926643.
  • Krzyscik MA, Porębska N, Opaliński Ł, Otlewski J. Targeting HER2 and FGFR-positive cancer cells with a bispecific cytotoxic conjugate combining anti-HER2 Affibody and FGF2. Int J Biol Macromol. 2024 Jan;254(Pt 1):127657. doi: 10.1016/j.ijbiomac.2023.127657. Epub 2023 Oct 28. PMID: 38287563.
  • Biadun M, Karelus R, Krowarsch D, Opalinski L, Zakrzewska M. FGF12: biology and function. Differentiation. 2024 Sep-Oct;139:100740. doi: 10.1016/j.diff.2023.100740. Epub 2023 Nov 25. PMID: 38042708.
  • Lampart A, Krowarsch D, Biadun M, Sorensen V, Szymczyk J, Sluzalska K, Wiedlocha A, Otlewski J, Zakrzewska M. Intracellular FGF1 protects cells from apoptosis through direct interaction with p53. Cell Mol Life Sci. 2023 Oct 2;80(10):311. doi: 10.1007/s00018-023-04964-9. PMID: 37783936; PMCID: PMC10545594.
  • Szymczyk J, Czyrek A, Otlewski J, Zakrzewska M. FGF1 Protects MCF-7 Cells against Taltobulin through Both the MEKs/ERKs and PI3K/AKT Signaling Pathway. Biomedicines. 2023 Jun 29;11(7):1856. doi: 10.3390/biomedicines11071856. PMID: 37509496; PMCID: PMC10376943.
  • Gregorczyk P, Porębska N, Żukowska D, Chorążewska A, Gędaj A, Malinowska A, Otlewski J, Zakrzewska M, Opaliński Ł. N-glycosylation acts as a switch for FGFR1 trafficking between the plasma membrane and nuclear envelope. Cell Commun Signal. 2023 Jul 21;21(1):177. doi: 10.1186/s12964-023-01203-3. PMID: 37480072; PMCID: PMC10362638.
  • Porębska N, Ciura K, Chorążewska A, Zakrzewska M, Otlewski J, Opaliński Ł. Multivalent protein-drug conjugates – An emerging strategy for the upgraded precision and efficiency of drug delivery to cancer cells. Biotechnol Adv. 2023 Oct;67:108213. doi: 10.1016/j.biotechadv.2023.108213. Epub 2023 Jul 13. PMID: 37453463.
  • Nawrocka D, Krzyscik MA, Sluzalska KD, Otlewski J. Dual-Warhead Conjugate Based on Fibroblast Growth Factor 2 Dimer Loaded with α-Amanitin and Monomethyl Auristatin E Exhibits Superior Cytotoxicity towards Cancer Cells Overproducing Fibroblast Growth Factor Receptor 1. Int J Mol Sci. 2023 Jun 14;24(12):10143. doi: 10.3390/ijms241210143. PMID: 37373291; PMCID: PMC10299504.
  • Biadun M, Sochacka M, Karelus R, Baran K, Czyrek A, Otlewski J, Krowarsch D, Opalinski L, Zakrzewska M. FGF homologous factors are secreted from cells to induce FGFR-mediated anti-apoptotic response. FASEB J. 2023 Jul;37(7):e23043. doi: 10.1096/fj.202300324R. PMID: 37342898.
  • Gedaj A, Zukowska D, Porebska N, Pozniak M, Krzyscik M, Czyrek A, Krowarsch D, Zakrzewska M, Otlewski J, Opalinski L. Correction: Galectins use N-glycans of FGFs to capture growth factors at the cell surface and fine-tune their signaling. Cell Commun Signal. 2023 Jun 13;21(1):129. doi: 10.1186/s12964-023-01192-3. Erratum for: Cell Commun Signal. 2023 May 25;21(1):122. doi: 10.1186/s12964-023-01144-x. PMID: 37312124; PMCID: PMC10262509.
  • Gedaj A, Zukowska D, Porebska N, Pozniak M, Krzyscik M, Czyrek A, Krowarsch D, Zakrzewska M, Otlewski J, Opalinski L. Short report galectins use N-glycans of FGFs to capture growth factors at the cell surface and fine-tune their signaling. Cell Commun Signal. 2023 May 25;21(1):122. doi: 10.1186/s12964-023-01144-x. Erratum in: Cell Commun Signal. 2023 Jun 13;21(1):129. doi: 10.1186/s12964-023-01192-3. PMID: 37231412; PMCID: PMC10214663.
  • Zukowska D, Gedaj A, Porebska N, Pozniak M, Krzyscik M, Czyrek A, Krowarsch D, Zakrzewska M, Otlewski J, Opalinski L. Receptor clustering by a precise set of extracellular galectins initiates FGFR signaling. Cell Mol Life Sci. 2023 Apr 3;80(4):113. doi: 10.1007/s00018-023-04768-x. PMID: 37012400; PMCID: PMC10070233.
  • Gonet M, Krowarsch D, Schubert J, Tabiś A, Bania J. Stability and Resistance to Proteolysis of Enterotoxins SEC and SEL Produced by Staphylococcus epidermidis and Staphylococcus aureus. Foodborne Pathog Dis. 2023 Jan;20(1):32-37. doi: 10.1089/fpd.2022.0059. PMID: 36622956.
  • Sochacka M, Karelus R, Opalinski L, Krowarsch D, Biadun M, Otlewski J, Zakrzewska M. FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex. Cell Commun Signal. 2022 Nov 21;20(1):182. doi: 10.1186/s12964-022-01000-4. PMID: 36411431; PMCID: PMC9677703.
  • Szymczyk J, Sochacka M, Chudy P, Opalinski L, Otlewski J, Zakrzewska M. FGF1 protects FGFR1-overexpressing cancer cells against drugs targeting tubulin polymerization by activating AKT via two independent mechanisms. Front Oncol. 2022 Oct 6;12:1011762. doi: 10.3389/fonc.2022.1011762. PMID: 36276073; PMCID: PMC9582358.
  • Poźniak M, Żukowska D, Gędaj A, Krzyścik MA, Porębska N, Zakrzewska M, Otlewski J, Opaliński Ł. The Significance of Cell Surface N-Glycosylation for Internalization and Potency of Cytotoxic Conjugates Targeting Receptor Tyrosine Kinases. Int J Mol Sci. 2022 Jul 31;23(15):8514. doi: 10.3390/ijms23158514. PMID: 35955648; PMCID: PMC9368766.
  • Krzyscik MA, Opaliński Ł, Szymczyk J, Otlewski J. Cyclic and dimeric fibroblast growth factor 2 variants with high biomedical potential. Int J Biol Macromol. 2022 Oct 1;218:243-258. doi: 10.1016/j.ijbiomac.2022.07.105. Epub 2022 Jul 22. PMID: 35878661.
  • Jendryczko K, Rzeszotko J, Krzyscik MA, Kocyła A, Szymczyk J, Otlewski J, Szlachcic A. Drug Conjugation via Maleimide-Thiol Chemistry Does Not Affect Targeting Properties of Cysteine-Containing Anti-FGFR1 Peptibodies. Mol Pharm. 2022 May 2;19(5):1422-1433. doi: 10.1021/acs.molpharmaceut.1c00946. Epub 2022 Apr 7. PMID: 35389227; PMCID: PMC9066409.
  • Lampart A, Sluzalska KD, Czyrek A, Szerszen A, Otlewski J, Wiedlocha A, Zakrzewska M. Nuclear Localization Sequence of FGF1 Is Not Required for Its Intracellular Anti-Apoptotic Activity in Differentiated Cells. Cells. 2022 Feb 2;11(3):522. doi: 10.3390/cells11030522. PMID: 35159330; PMCID: PMC8833943.
  • Jendryczko K, Rzeszotko J, Krzyscik MA, Szymczyk J, Otlewski J, Szlachcic A. Peptibody Based on FGFR1-Binding Peptides From the FGF4 Sequence as a Cancer-Targeting Agent. Front Pharmacol. 2021 Nov 12;12:748936. doi: 10.3389/fphar.2021.748936. PMID: 34867353; PMCID: PMC8636100.
  • Porębska N, Knapik A, Poźniak M, Krzyścik MA, Zakrzewska M, Otlewski J, Opaliński Ł. Intrinsically Fluorescent Oligomeric Cytotoxic Conjugates Toxic for FGFR1-Overproducing Cancers. Biomacromolecules. 2021 Dec 13;22(12):5349-5362. doi: 10.1021/acs.biomac.1c01280. Epub 2021 Dec 2. PMID: 34855396; PMCID: PMC8672352.
  • Szymczyk J, Sluzalska KD, Materla I, Opalinski L, Otlewski J, Zakrzewska M. FGF/FGFR-Dependent Molecular Mechanisms Underlying Anti-Cancer Drug Resistance. Cancers (Basel). 2021 Nov 18;13(22):5796. doi: 10.3390/cancers13225796. PMID: 34830951; PMCID: PMC8616288.
  • Poźniak M, Porębska N, Jastrzębski K, Krzyścik MA, Kucińska M, Zarzycka W, Barbach A, Zakrzewska M, Otlewski J, Miączyńska M, Opaliński Ł. Modular self-assembly system for development of oligomeric, highly internalizing and potent cytotoxic conjugates targeting fibroblast growth factor receptors. J Biomed Sci. 2021 Oct 11;28(1):69. doi: 10.1186/s12929-021-00767-x. PMID: 34635096; PMCID: PMC8504119.
  • Wiedlocha A, Haugsten EM, Zakrzewska M. Roles of the FGF-FGFR Signaling System in Cancer Development and Inflammation. Cells. 2021 Aug 28;10(9):2231. doi: 10.3390/cells10092231. PMID: 34571880; PMCID: PMC8471549.
  • Krzyscik MA, Zakrzewska M, Sørensen V, Øy GF, Brunheim S, Haugsten EM, Mælandsmo GM, Wiedlocha A, Otlewski J. Fibroblast Growth Factor 2 Conjugated with Monomethyl Auristatin E Inhibits Tumor Growth in a Mouse Model. Biomacromolecules. 2021 Oct 11;22(10):4169-4180. doi: 10.1021/acs.biomac.1c00662. Epub 2021 Sep 20. PMID: 34542998; PMCID: PMC8512659.
  • Poźniak M, Zarzycka W, Porębska N, Knapik A, Marczakiewicz-Perera P, Zakrzewska M, Otlewski J, Opaliński Ł. FGF1 Fusions with the Fc Fragment of IgG1 for the Assembly of GFPpolygons-Mediated Multivalent Complexes Recognizing FGFRs. Biomolecules. 2021 Jul 23;11(8):1088. doi: 10.3390/biom11081088. PMID: 34439755; PMCID: PMC8392455.
  • Poźniak M, Porębska N, Krzyścik MA, Sokołowska-Wędzina A, Jastrzębski K, Sochacka M, Szymczyk J, Zakrzewska M, Otlewski J, Opaliński Ł. The cytotoxic conjugate of highly internalizing tetravalent antibody for targeting FGFR1-overproducing cancer cells. Mol Med. 2021 May 7;27(1):46. doi: 10.1186/s10020-021-00306-2. PMID: 33962559; PMCID: PMC8103757.
  • Porębska N, Poźniak M, Matynia A, Żukowska D, Zakrzewska M, Otlewski J, Opaliński Ł. Galectins as modulators of receptor tyrosine kinases signaling in health and disease. Cytokine Growth Factor Rev. 2021 Aug;60:89-106. doi: 10.1016/j.cytogfr.2021.03.004. Epub 2021 Mar 27. PMID: 33863623.
  • Porebska N, Pozniak M, Krzyscik MA, Knapik A, Czyrek A, Kucinska M, Jastrzebski K, Zakrzewska M, Otlewski J, Opalinski L. Dissecting biological activities of fibroblast growth factor receptors by the coiled-coil-mediated oligomerization of FGF1. Int J Biol Macromol. 2021 Jun 1;180:470-483. doi: 10.1016/j.ijbiomac.2021.03.094. Epub 2021 Mar 18. PMID: 33745974.
  • Krzyscik MA, Sokolowska-Wedzina A, Jendryczko K, Pozniak M, Nawrocka D, Porebska N, Zakrzewska M, Otlewski J, Szlachcic A, Opalinski L. Preparation of Site-Specific Cytotoxic Protein Conjugates via Maleimide-thiol Chemistry and Sortase A-Mediated Ligation. J Vis Exp. 2021 Jan 5;(167). doi: 10.3791/61918. PMID: 33491672.
  • Jendryczko K, Chudzian J, Skinder N, Opaliński Ł, Rzeszótko J, Wiedlocha A, Otlewski J, Szlachcic A. FGF2-Derived PeptibodyF2-MMAE Conjugate for Targeted Delivery of Cytotoxic Drugs into Cancer Cells Overexpressing FGFR1. Cancers (Basel). 2020 Oct 15;12(10):2992. doi: 10.3390/cancers12102992. PMID: 33076489; PMCID: PMC7602595.
  • Nawrocka D, Krzyscik MA, Opaliński Ł, Zakrzewska M, Otlewski J. Stable Fibroblast Growth Factor 2 Dimers with High Pro-Survival and Mitogenic Potential. Int J Mol Sci. 2020 Jun 9;21(11):4108. doi: 10.3390/ijms21114108. PMID: 32526859; PMCID: PMC7312490.
  • Pozniak M, Sokolowska-Wedzina A, Jastrzebski K, Szymczyk J, Porebska N, Krzyscik MA, Zakrzewska M, Miaczynska M, Otlewski J, Opalinski L. FGFR1 clustering with engineered tetravalent antibody improves the efficiency and modifies the mechanism of receptor internalization. Mol Oncol. 2020 Sep;14(9):1998-2021. doi: 10.1002/1878-0261.12740. Epub 2020 Jul 3. PMID: 32511887; PMCID: PMC7463352.
  • Pozniak M, Sokolowska-Wedzina A, Jastrzebski K, Szymczyk J, Porebska N, Krzyscik MA, Zakrzewska M, Miaczynska M, Otlewski J, Opalinski L. FGFR1 clustering with engineered tetravalent antibody improves the efficiency and modifies the mechanism of receptor internalization. Mol Oncol. 2020 Sep;14(9):1998-2021. doi: 10.1002/1878-0261.12740. Epub 2020 Jul 3. PMID: 32511887; PMCID: PMC7463352.
  • Duda P, Wójtowicz T, Janczara J, Krowarsch D, Czyrek A, Gizak A, Rakus D. Fructose 1,6-Bisphosphatase 2 Plays a Crucial Role in the Induction and Maintenance of Long-Term Potentiation. Cells. 2020 Jun 1;9(6):1375. doi: 10.3390/cells9061375. PMID: 32492972; PMCID: PMC7349836.
  • Sluzalska KD, Slawski J, Sochacka M, Lampart A, Otlewski J, Zakrzewska M. Intracellular partners of fibroblast growth factors 1 and 2 – implications for functions. Cytokine Growth Factor Rev. 2021 Feb;57:93-111. doi: 10.1016/j.cytogfr.2020.05.004. Epub 2020 May 18. PMID: 32475760.
  • Sochacka M, Opalinski L, Szymczyk J, Zimoch MB, Czyrek A, Krowarsch D, Otlewski J, Zakrzewska M. FHF1 is a bona fide fibroblast growth factor that activates cellular signaling in FGFR-dependent manner. Cell Commun Signal. 2020 May 1;18(1):69. doi: 10.1186/s12964-020-00573-2. PMID: 32357892; PMCID: PMC7193404.
  • Szlachcic A, Sochacka M, Czyrek A, Opalinski L, Krowarsch D, Otlewski J, Zakrzewska M. Low Stability of Integrin-Binding Deficient Mutant of FGF1 Restricts Its Biological Activity. Cells. 2019 Aug 15;8(8):899. doi: 10.3390/cells8080899. PMID: 31443196; PMCID: PMC6721657.
  • Krzyscik MA, Opaliński Ł, Otlewski J. Novel Method for Preparation of Site-Specific, Stoichiometric-Controlled Dual Warhead Conjugate of FGF2 via Dimerization Employing Sortase A-Mediated Ligation. Mol Pharm. 2019 Aug 5;16(8):3588-3599. doi: 10.1021/acs.molpharmaceut.9b00434. Epub 2019 Jun 24. PMID: 31244217.
  • Kucińska M, Porębska N, Lampart A, Latko M, Knapik A, Zakrzewska M, Otlewski J, Opaliński Ł. Differential regulation of fibroblast growth factor receptor 1 trafficking and function by extracellular galectins. Cell Commun Signal. 2019 Jun 17;17(1):65. doi: 10.1186/s12964-019-0371-1. PMID: 31208421; PMCID: PMC6572767.
  • Latko M, Czyrek A, Porębska N, Kucińska M, Otlewski J, Zakrzewska M, Opaliński Ł. Cross-Talk between Fibroblast Growth Factor Receptors and Other Cell Surface Proteins. Cells. 2019 May 14;8(5):455. doi: 10.3390/cells8050455. PMID: 31091809; PMCID: PMC6562592.
  • Lipok M, Szlachcic A, Kindela K, Czyrek A, Otlewski J. Identification of a peptide antagonist of the FGF1-FGFR1 signaling axis by phage display selection. FEBS Open Bio. 2019 May;9(5):914-924. doi: 10.1002/2211-5463.12618. Epub 2019 Apr 9. PMID: 30968602; PMCID: PMC6487701.
  • Porębska N, Latko M, Kucińska M, Zakrzewska M, Otlewski J, Opaliński Ł. Targeting Cellular Trafficking of Fibroblast Growth Factor Receptors as a Strategy for Selective Cancer Treatment. J Clin Med. 2018 Dec 20;8(1):7. doi: 10.3390/jcm8010007. PMID: 30577533; PMCID: PMC6352210.
  • Chudzian J, Szlachcic A, Zakrzewska M, Czub M, Pustula M, Holak TA, Otlewski J. Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity. Int J Mol Sci. 2018 Aug 21;19(9):2470. doi: 10.3390/ijms19092470. PMID: 30134556; PMCID: PMC6163658.
  • Kostas M, Lampart A, Bober J, Wiedlocha A, Tomala J, Krowarsch D, Otlewski J, Zakrzewska M. Translocation of Exogenous FGF1 and FGF2 Protects the Cell against Apoptosis Independently of Receptor Activation. J Mol Biol. 2018 Oct 19;430(21):4087-4101. doi: 10.1016/j.jmb.2018.08.004. Epub 2018 Aug 9. PMID: 30099027.
  • Świderska KW, Szlachcic A, Opaliński Ł, Zakrzewska M, Otlewski J. FGF2 Dual Warhead Conjugate with Monomethyl Auristatin E and α-Amanitin Displays a Cytotoxic Effect towards Cancer Cells Overproducing FGF Receptor 1. Int J Mol Sci. 2018 Jul 19;19(7):2098. doi: 10.3390/ijms19072098. PMID: 30029518; PMCID: PMC6073801.
  • Świderska KW, Szlachcic A, Opaliński Ł, Zakrzewska M, Otlewski J. FGF2 Dual Warhead Conjugate with Monomethyl Auristatin E and α-Amanitin Displays a Cytotoxic Effect towards Cancer Cells Overproducing FGF Receptor 1. Int J Mol Sci. 2018 Jul 19;19(7):2098. doi: 10.3390/ijms19072098. PMID: 30029518; PMCID: PMC6073801.
  • Opaliński Ł, Szymczyk J, Szczepara M, Kucińska M, Krowarsch D, Zakrzewska M, Otlewski J. High Affinity Promotes Internalization of Engineered Antibodies Targeting FGFR1. Int J Mol Sci. 2018 May 10;19(5):1435. doi: 10.3390/ijms19051435. PMID: 29748524; PMCID: PMC5983756.
  • Borek A, Sokolowska-Wedzina A, Chodaczek G, Otlewski J. Generation of high-affinity, internalizing anti-FGFR2 single-chain variable antibody fragment fused with Fc for targeting gastrointestinal cancers. PLoS One. 2018 Feb 8;13(2):e0192194. doi: 10.1371/journal.pone.0192194. PMID: 29420662; PMCID: PMC5805272.

Projekt "Zintegrowany Program Rozwoju Uniwersytetu Wrocławskiego 2018-2022" współfinansowany ze środków Unii Europejskiej z Europejskiego Funduszu Społecznego